1000 users is the minimum number of users a remarketing list must have before being used with a Remarketing List for Search Ads campaign.

Google Search Network must have a minimum of 1,000 active visitors or users within the last 30 days

A remarketing list is a collection of website visitors or app users gathered by snippets of code added to your site or app. If you’ve set up the Google Ads tag for remarketing in the new Google Ads experience, you’ll see the new Google Ads tag that consists of a global site tag and an optional event snippet.

Learn more here: https://support.google.com/google-ads/answer/2472738

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